Tuberculosis vaccine

ABSTRACT

The present invention relates to novel recombinant vaccines providing protective immunity against tuberculosis. Further, the present invention refers to novel recombinant nucleic acid molecules, vectors containing said nucleic acid molecules, cells transformed with said nucleic acid molecules and polypeptides encoded by said nucleic acid molecules.

[0001] The present invention relates to novel recombinant vaccines providing protective immunity especially against tuberculosis. Further, the present invention refers to novel recombinant nucleic acid molecules, vectors containing said nucleic acid molecules, cells transformed with said nucleic acid molecules and polypeptides encoded by said nucleic acid molecules.

[0002] Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a significant global problem. It is estimated that one third of the world's population is infected with M. tuberculosis (Kochi, 1991). In many countries the only measure for TR control has been vaccination with M. bovis bacille Calmette-Guérin (BCG). The overall vaccine efficacy of BCG against TB, however, is about 50% with extreme variations ranging from 0% to 80% between different field trials (Roche et al., 1995). Thus, BCG should be improved, e.g. by genetic engineering, to provide a vaccine for better TB control (Murray et al., 1996; Hess and Kaufmann, 1993). The widespread emergence of multiple drug-resistant M. tuberculosis strains additionally underlines the urgent requirement for novel TB vaccines (Grange, 1996).

[0003]M. tuberculosis belongs to the group of intracellular bacteria that replicate within the phagosomal vacuoles of resting macrophages, thus protection against TB depends on T cell-mediated immunity (Kaufmann, 1993). Several studies in mice and humans, however, have shown that mycobacteria stimulate antigen-specific, major histocompatibility complex (MHC) class II- or class I-restricted CD4 and CD8 T cells, respectively (Kaufmann, 1993).

[0004] The important role of MHC class i-restricted CD8 T cells was convincingly demonstrated by the failure of β2-microglobulin (β2m) deficient mice to control experimental M. tuberculosis infection (Flynn et al., 1993). Because these mutant mice lack MHC class I, functional CD8 T cells cannot develop. In contrast to M. tuberculosis infection, β2m-deficient mice are capable of controlling certain infectious doses of the BCG vaccine strain (Flynn et al., 1993; Ladel et al., 1995). Furthermore, BCG vaccination of β2m-deficient mice prolonged survival after subsequent M. tuberculosis infection whereas BCG-immunized C57BL/6 resisted TB (Flynn et al., 1993). This differential CD8 T cell dependency between M. tuberculosis and BCG may be explained as follows. M. tuberculosis antigens gain better access to the cytoplasm than antigens from BCG leading to more pronounced MHC class I presentation (Hess and Kaufmann, 1993). Consequently, a more effective CD8 T cell response is generated by M. tuberculosis. This notion was recently supported by increased MHC class I presentation of an irrelevant antigen, ovalbumin, by simultaneous M. tuberculosis, rather than BCG, infection of antigen presenting cells (APC) (Mazzaccaro et al., 1996).

[0005] Secreted proteins of M. tuberculosis comprise a valuable source of antigens for MHC class I presentation. Recently, a DNA vaccine encoding the secreted antigen Ag85A elicited MHC class I-restricted CD8 T cell responses in mice which may contribute to defence against TB (Huygen et al., 1996). In general, evidence is accumulating that immunization with secreted protein antigens of M. tuberculosis induce some protection against TB in guinea pigs and mice (Horwitz et al., 1995; Andersen, 1994). An important goal towards the development of improved TB vaccines based on BCG, therefore, is to augment the accessibility of secreted BCG-specific antigens to the cytoplasm of infected APC. Subsequent delivery of peptides derived from these secreted proteins into the MHC class I presentation pathway may potentiate the already existing BCG-specific immune response for preventing TB.

[0006] The phagolysosomal escape of L. monocytogenes represents a unique mechanism to facilitate MHC class I antigen presentation of listerial antigens (Berche et al., 1987; Portnoy et al., 1988). Listeriolysin (Hly), a pore-forming sulfhydryl-activated cytolysin, is essential for the release of L. monocytogenes microorganisms from phagolysosomal vacuoles into the cytosol of host cells (Gaillard at al., 1987, Portnoy et al., 1988). This escape function was recently transferred to Bacillus subtilis and to attenuated Salmonella ssp. strains (Bielecki et al., 1991, Gentschev et al., 1995; Hess and Kaufmann, 1997). Hly expression by an asporogenic B. subtilis mutant strain or in Salmonella ssp, results in bacterial escape from the phagolysosome into the cytosol of J774 macrophage-like cells (Bielecki et al., 1991; Gentschev et al., 1995; Hess and Kaufmann, 1997).

[0007] Thus, the transfer of lysosomal escape functions to heterologous microorganisms may cause an elevated toxicity of the resulting recombinant microorganisms. For this reason, the use of these lysosomal escape functions for the preparation of recombinant living vaccines has not been readily taken into consideration.

[0008] According to the present invention recombinant BCG strains secreting hemolytically active Hly were constructed which show an improved efficacy MHC class I-restricted immune response and, surprisingly, an equal or even lower cytotoxicity in comparison with the unmodified native BCG strains. Thus, these recombinant organisms are promising candidate vaccines against TB.

[0009] A first aspect of the present invention is a recombinant nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is capable of eliciting an immune response in a mammal, and (b) a phagolysosomal escape domain.

[0010] A specific embodiment of this first aspect is the nucleic acid molecule in SEQ ID No.1. This nucleic acid molecule comprises a signal peptide coding sequence (nucleotide 1-120), a sequence coding for an immunogenic domain (nucleotide 121-153), a peptide linker coding sequence (nucleotide 154-210), a sequence coding for a phagolysosomal domain (nucleotide 211-1722), a further peptide linker coding sequence (nucleotide 1723-1800) and a sequence coding for a random peptide (nucleotide 1801-1870), The corresponding amino acid sequence is shown in SEQ ID No.2.

[0011] The nucleic acid of the present invention contains at least one immunogenic domain from a polypeptide derived from an organism of the genus Mycobacterium, preferably from Mycobacterium tuberculosis or from Mycobacterium bovis. This domain has a length of at least 6, preferably of at least 8 amino acids. The immunogenic domain is preferably a portion of a native Mycobacterium polypeptide. However, within the scope of the present invention is also a modified immunogenic domains which is derived from a native immunogenic domain by substituting, deleting and/or adding one or several amino acids.

[0012] The immunogenic domain is capable of eliciting an immune response in a mammal, This immune response can be a B cell-mediated immune response. Preferably, however, the immunogenic domain is capable of eliciting a T cell-mediated immune response, more preferably a MHC class I-restricted CD8 T cell response.

[0013] The domain capable of eliciting an immune response is peferably selected from immunogenic peptides or polypeptides from M. bovis or M. tuberculosis or from immunogenic fragments thereof. Specific examples for suitable so antigens are Ag85B (p30) from M. tuberculosis (Harth et al., 1996), Ag85B (α-antigen) from M. bovis BCG (Matsuo et al, 1988), Ag85A from M. tuberculosis (Huygen et al., 1996) and ESAT-6 from M. tuberculosis (Sorensen et al., 1996, Harboe et al., 1996 and Andersen et al., 1995). More preferably, the immunogenic domain is derived from the antigen Ag85B. Most preferably, the immunogenic domain comprises the sequence from aa.41 to aa.51 in SEQ ID No.2.

[0014] The recombinant nucleic acid molecule according to the present invention further comprises a phagolysosomal escape domain, i.e. a polypeptide domain which provides for an escape of the fusion polypeptide from the phagolysosome into the cytosol of mammalian cells. Preferably, the phagolysosomal escape domain is derived from an organism of the genus Listeria. More preferably, the phagolysosomal escape domain is derived from the organism L. monocytogenes. Most preferably, the phagolysosomal domain is encoded by a nucleic acid molecule selected from: (a) the nucleotide sequence from nucleotide 211-1722 as shown in SEQ ID No.1, (b) a nucleotide sequence which encodes for the same amino acid sequence as the sequence from (a), and (c) a nucleotide sequence hybridizing under stringent conditions with the sequence from (a) or (b).

[0015] Apart from the nucleotide sequence depicted in SEQ ID No.1 the present invention also comprises nucleic acid sequences hybridizing therewith. In the present invention the term “hybridization” is used as defined in Sambrook et al. (Molecular Cloning. A laboratory manual, Cold Spring Harbor Laboratory Press (19891, 1.101-1.104). In accordance with the present invention the term “hybridization” is used if a positive hybridization signal can still be observed after washing for one hour with 1×SSC and 0.1% SDS at 55° C., preferably at 62° C. and more preferably at 68° C., particularly for 1 hour in 0.2×SSC and 0.1% SDS at 55° C., preferably at 62° C. and more preferably at 68° C. A sequence hybridizing with a nucleotide sequence as per SEQ ID No.1 under such washing conditions is a phagolysosomal escape domain encoding nucleotide sequence preferred by the subject invention.

[0016] Preferably, the recombinant nucleic acid molecule encoding for a fusion polypeptide contains a signal peptide encoding sequence. More preferably, the signal sequence is a signal sequence active in Mycobacteria, preferably in M. bovis, e.g. a native M. bovis signal sequence. A preferred example of a suitable signal sequence is the nucleotide sequence coding for the Ag85B signal peptide which is depicted in SEQ ID No.1 from nucleotide 1 to 120.

[0017] Further, it is preferred that a peptide linker be provided between the immunogenic domain and the phagolysosomal escape domain. Preferably, said peptide linker has a length of from 5 to 50 amino acids. More preferably, a sequence encoding a linker as shown in SEQ ID No.1 from nucleotide 154 to 210 or a sequence corresponding thereto as regards the degeneration of the genetic code.

[0018] A further subject matter of the invention pertains to a recombinant vector comprising at least one copy of a nucleic acid molecule as defined above. Preferably, the recombinant vector is a prokaryotic vector, i.e. a vector containing elements for replication or/and genomic integration in prokaryotic cells. Preferably, the recombinant vector carries the nucleic acid molecule of the present invention operatively linked with an expression control sequence. The expression control sequence is preferably an expression control sequence active in Mycobacteria, particularly in M. bovis. The vector can be an extrachromosomal vector or a vector suitable for integration into the chromosome. Examples of such vectors are known to the man skilled in the art and, for instance, given in Sambrook et al. supra.

[0019] A still further subject matter of the invention is a cell comprising a recombinant nucleic acid molecule or a vector as defined above. Preferably, the cell is prokaryotic, particularly a Mycobacterium cell, Further, it is preferred that the cell is capable of expressing the nucleic acid molecule of the invention.

[0020] In a second aspect of the present invention a recombinant Mycobacterium bovis cell is provided which comprises at least one recombinant nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain capable of eliciting an immune response in a mammal and (b) a phagolysosomal escape domain. According to this aspect, the immunogenic domain is not restricted to Mycobacterium antigens and can be selected from autoantigens, tumor antigens and pathogen antigens such as virus antigens, parasite antigens, bacterial antigens in general and immunogenic fragments thereof. Specific examples for suitable tumor antigens are human tumor antigens such as the p53 tumor suppressor gene product (Houbiers et al., 1993) and melanocyte differentiation antigens, e.g. Melan-A/MART-1 and gp100 (van Elsas et al., 1996). Specific examples for suitable virus antigens are human tumor virus antigens such as human papilloma virus antigens, e.g. antigens E6 and E7 (Bosch et al., 1991), influenza virus antigens, e.g. influenza virus nucleoprotein (Matsui et al., 1995; Fu et al., 1997) or retroviral antigens such as HIV antigens, e.g. the HIV-1 antigens p17, p24, RT and Env (Harrer et al., 1996; Haas et al., 1996). Specific examples for suitable parasite antigens are Plasmodium antigens such as liver stage antigen (LSA-1), circumsporozoite protein (CS or allelic variants cp26 or cp29), thrombospondin related amonymous protein (TRAP), sporozoite threonine and asparagine rich protein (STARP) from Plasmodium falciparum (Aidoo et al., 1995) and Toxoplasma antigens such as p30 from Toxoplasma gondii (Khan et al., 1991; Bulow and Boothroyd, 1991). Specific examples for suitable bacterial antigens are Legionella antigens such as Major secretary protein from Legionella pneumophila (Blander and Horwitz, 1991).

[0021] The cell according to the invention is preferably capable of secreting the fusion polypeptide encoded by the nucleic acid molecule of the invention and of providing it in a form suitable for MHC class I-restricted antigen recognition.

[0022] In a third aspect of the present invention a recombinant Mycobacterium bovis cell is provided which comprises at least one nucleic acid molecule encoding a phagolysosomal escape peptide or polypeptide. Even if the phagolysosomal escape peptide or polypeptide is not fusioned with an antigen, a surprising improvement of the immunogenic properties is found.

[0023] The recombinant Mycobacterium bovis cell which is provided according to the present invention may contain at least one further recombinant, e.g. heterologous nucleic acid molecule encoding a peptide or polypeptide capable of eliciting an immune response in a mammal. Said further immunogenic peptide or polypeptide may be selected from Mycobacterium antigens or, in a wider sense, from autoantigens, tumor antigens, pathogen antigens and immunogenic fragments thereof. The nucleic acid molecule coding for the further peptide or polypeptide may be situated on the same vector as the fusion gene. However, it may, for example, also be situated on a different plasmid, independently of the fusion gene, or be chromosomally integrated.

[0024] Surprisingly, it was found that a Mycobacterium cell according to the present invention has an intracellular persistence in infected cells, e.g. macrophages, which is equal or less than the intracellular persistence of a corresponding native Mycobacterium cell which does not contain the recombinant nucleic acid molecule.

[0025] A still further subject matter of the present invention is a recombinant fusion polypeptide encoded by a nucleic acid molecule as defined above. The fusion polypeptide according to the invention imparts to a cell the capability of improved MHC class I-restricted antigen recognition.

[0026] The present invention also refers to a pharmaceutical composition comprising as an active agent a cell or a fusion polypeptide as defined above, optionally together with pharmaceutically acceptable diluents, carriers and adjuvants. Preferably, the composition is a living vaccine suitable for administration to a mammal, preferably a human, The actually chosen vaccination route depends on the choice of the vaccination vector. Administration may be achieved in a single dose or repeated at intervals. The appropriate dosage depends on various parameters such as the vaccinal vector itself or the route of administration. Administration to a mucosal surface (e.g. ocular, intranasal, oral, gastric, intestinal, rectal, vaginal or urinary tract) or via the parenteral route (e.g. subcutaneous, intradermal, intramuscular, intravenous or intraperitoneal) might be chosen.

[0027] Further, the present invention pertains to a method for preparing a recombinant bacterial cell as defined above. According to the first aspect, this method comprises the steps of (i) inserting a recombinant nucleic acid molecule into a bacterial cell, said nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a Mycobacterium polypeptide wherein said domain is capable of eliciting an immune response in a mammal and (b) a phagolysosomal escape domain, and (ii) cultivating the cell obtained according to step (i) under suitable conditions, Preferably, a cell is obtained which is capable of expressing said nucleic acid molecule. Preferably, the cell is a M. bovis cell.

[0028] According to the second aspect, this method comprises the steps of (i) inserting a recombinant nucleic acid molecule into a Mycobacterium bovis cell, said nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a polypeptide, wherein said domain is capable of eliciting an immune response in a mammal, and (b) a phagolysosomal escape domain, and (ii) cultivating the cell obtained according to (i) under suitable conditions.

[0029] According to the third aspect, this method comprises the step of (i) inserting a recombinant nucleic acid molecule into a Mycobacterium bovis cell, said nucleic acid molecule encoding a phagolysosomal escape peptide or polypeptide, and (ii) cultivating the cell obtained according to (i) under suitable conditions.

[0030] If desired, the method of the present invention comprises inserting at least one further recombinant nucleic acid molecule into the Mycobacterium bovis cell, said further recombinant nucleic acid molecule encoding a peptide or polypeptide capable of eliciting an immune response in a mammal.

[0031] Finally, the present invention relates to a method for the preparation of a living vaccine comprising formulating the recombinant cell in a pharmaceutically effective amount with pharmaceutically acceptable diluents, carriers and/or adjuvants.

[0032] The invention will be further illustrated by the following figures and sequence listings.

[0033]FIG. 1: shows plasmid maps for Hly secretion by recombinant BCG strains.

[0034] A. Extrachromosomal Hly expression by Escherichia coli-mycobacteria shuttle plasmid pAT261:Hly. Insertion of the pILH-1-derived 1.7 kb Pst I-fragment encoding the DNA sequence of the mature Hly protein. Abbreviations: Mrep, mycobacterial replicon; Erep, E. coli origin of replication; kan, kanamycin-resistance gene; hsp, heat shock protein promoter.

[0035] B. Chromosomal integrative shuttle vector pMV306:Hly for Hly expression by mycobacteria. The inserted DNA-restriction fragment (Xba I-Sal I) including the hsp60 promoter is derived from plasmid PAT261:Hly. Abbreviations: attP, attachment site of mycobacteriophage L5; MCS, multiple cloning site; int, integrase of mycobacteriophage L5.

[0036]FIG. 2: shows the amino acid sequence of the Hly fusion expressed by BCG pAT261; Hly or BCG pMV306:Hly. The amino acid sequence corresponding to the hly gene-specific open reading frame is derived from the DNA sequence of the mycobacteria expression plasmids pAT261:Hly or pMV306:Hly. The Hly fusion protein consists of the following different polypeptide sequences: BCG-specific Ag85B including signal peptide, underlined amino acid sequence in single letter code, (previously termed αantigen; Matsuo et al., 1988); E. coli pHly152-specific HlyA, italic letters, (Hess et al., 1986); mature Hly, bold letters, (Domann and Chakraborty, 1989); random amino acid sequence, normal letters. The used restriction sites (Pst I and Nst I) for corresponding gene fusions are presented below the amino acid sequence,

[0037]FIG. 3: shows the analysis of Hly expression by recombinant BCG. Detection of Hly fusion protein in lysates (L) or supernatants (S) of BCG, BCG pAT261:Hly or BCG pMV306:Hly strains by immunostaining. Culture lysates and enriched supernatants of the different mycobacterial strains were separated on SDS/10% polyacrylamide gel and transferred to Hybond-PVDF membrane. The primary antibody used for chemiluminescent immunostaining of the 62 kDa Hly hybrid protein was anti-Hly mAb H14-3 (Nato et al, 1991).

[0038]FIG. 4: shows the intracellular growth and cytotoxicity of a recombinant BCG strain.

[0039] A. Survival of wild-type BCG (▪), BCG pAT261:Hly (Δ) and BCG pMV306,Hly (♦) strains in human macrophage-like cells THP-1.

[0040] B. Survival of wild-type BCG (▪), BCG pAT261:Hly (A) and BCG pMV306:Hly (♦) strains in murine J774A.1 macrophage-like cells. At 3 h post infection, r-BCG-specific. CFU were determined from infected-cell lysates and were monitored from day 0 to day 15. The data are presented as means±SD (n=3).

[0041] C. Supernatants and cell lysates of J774A.1 were assayed for LDH activity after BCG or r-BCG infection. J774A.1 (□), BCG (⋄), BCG pMV306:Hly (♦), BCG pAT261.Hly (Δ) or L. monocytogenes EGD (▪). Indicated is the cumulative percentage of total LDH activity detected in the supernatant (mean±SD). This is a representative experiment of three. The percent LDH released into the supernatant was determined as a measure of cell death.

[0042]FIG. 5: shows the induction of increased IFN-γ levels in mice immunized with the Hly secreting SC(3 strains pAT261-Hly and pMV306-Hly.

[0043]FIG. 6: shows the frequencies of IFN-γ producing CD8 T cells specific for mycobacterial antigens.

[0044]FIG. 7: shows the effects of Hly expression on the virulence of the recombinant BCG strains. The bacterial load on the lung of C57BL/6 (filled symbols) and TAP1 -deficient mice (open symbols) immunized with 10⁶ BCG (circles) or BCG pMV306-Hly (squares) i.v. was determined on day 100 and 150 post vaccination.

[0045]FIG. 8: shows the protective capacity of the Hly recombinant BCG strains in the aerosol model of murine tuberculosis.

[0046]FIG. 9: shows the protective capacity of the Hly recombinant BCG strains in the aerosol model of murine tuberculosis in a long term experiment.

[0047] A. Schematic representation of the experimental protocol.,

[0048] B. Bacterial load in infected organs (spleen, liver, lung) of one mouse strain (BALB/c) on day 30 post challenge,

[0049]FIG. 10: shows the protective capacity of Hly recombinant BCG strains in immunocompetent and deficient mice in the aerosol model in a long term experiment.

[0050] A. Schematic representation of the experimental protocol.

[0051] B. Bacterial load in infected organs (spleen, lung).

[0052] SEQ ID No.1: shows the nucleotide sequence of a nucleic acid molecule according to the present invention.

[0053] SEQ ID No.2: shows the corresponding amino acid sequence of the nucleic acid molecule of SEQ ID No.1.

EXAMPLES

[0054] 1. Experimental Procedures

[0055] 1.1 Bacterial Strains and Plasmids

[0056]M. bovis BCG strain Chicago (ATCC 27289) was cultured in Dubos broth base (Difco) supplemented with Dubos medium albumin (Difco) at 37° C. A mid-logarithmic culture was aliquoted and stored at −70° C. until use. L. monocytogenes EGD Sv 1/2a (Domann and Chakraborty, 1989) originally obtained from G. B. Mackaness was grown in brain heart infusion (BHI) broth (Difco) at 37° C. with aeration, Plasmid pILH-1 was a generous gift of Drs. I. Gentschev and W. Goebel (University of {umlaut over (Wurzburg)}, Germany). The mycobacteria—E. coli shuttle vectors DAT261 and QMV306 were obtained from Medimmune (Gaithersburg, U.S.A.).

[0057] 1.2 Enzymes and General Genetic Techniques

[0058] Restriction enzymes (Boehringer Mannheim) and T4 DNA ligase (Pharmacia) were used as recommended by the manufacturer. Molecular cloning and recombinant DNA techniques were performed following standard protocols (Sambrook ex al., 1989).

[0059] 1.3 DNA Manipulations and Sequencing

[0060] Extrachromosomal pAT261 (parental vector pAB261; Stover et al., 1993) and integrative pMV306 (parental vector pMV361; Stover et al., 1991) expression plasmids were used for Hly secretion. The plasmids DAT261 and pMV306 share common elements including an expression cassette, the Tn903-derived aph gene conferring kanamycin-resistance as a selectable marker, and an E. coli origin of replication derived from pUC19, They differ by the insertion of either a mycobacterial plasmid origin of replication (pAT261) or the attP and int genes of mycobacteriophage L5 (pMV306). The inserted DNA-fragment of the M. bovis BGC-specific Ag85B-gene in plasmid construct DAT261 is under the control of the BCG hsp60 promoter. The Pst I restriction site (position 4404, Medimmune) downstream the coding sequence for the mature Ag85B protein was used to construct Hly-derived fusions which maintain the hemolytic activity of native Hly from L. monocytogenes EGD and are exported by the N-terminal Ag85B-specific signal peptide. The 1.7 kb Pst I-fragment (original position 1357 and 4277; Hess et al., 1986) of the original gene fusion hly-hlyA encoded by plasmid pILH-1 (Gentschev et al., 1995; Hess et al., 1996) was used for constructing PAT261:Hly. The complete Xba I-Sal I DNA expression cassette, including hsp60 promoter, for the encoded Ag85B-Hly hybrid protein of plasmid pAT261 Hly was introduced into the plasmid pMV306. This resulting construct was termed pMV306:Hly. The correct DNA sequence of these plasmids at the sites of fragment insertion was determined using the following oligonucleotides BCG-Hly5-GCTTTGTCCTGCTG and BCG-Hly3-GGAAGTCAGGGTGA (Sequiserve, Vaterstetten, Germany). The DNA sequence analysis revealed a random insertion of a short Pst I-DNA fragment at the 3′-end of the hly-hlyA gene fusion which codes for 11 aa.

[0061] 1.4 Characterization of Recombinant M. bovis BCG Strains

[0062] The plasmids, DAT261:Hly or pMV306:Hly were introduced into M. bovis BCG strain Chicago by standard electroporation protocol (Langermann et al., 1994) and then recombinant colonies were selected on Middlebrook 7H10 agar supplemented with kanamycin (15 μg/ml). Kanamycin-resistant colonies were grown to mid-logarithmic phase in Dubos liquid media (Difco) containing 10% Dubos medium albumin (Difco) and 15 μg/ml kanamycin for three weeks. After washing the cells in phosphate buffered saline (PBS) plus Tween 80, the cell suspension was concentrated 20-fold in RIPA buffer (1% NP-40, 0.5% Deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) to lyse the cells. Bacteria-free supernatant (1 ml) of these cultures were filtered through 0.2-μm membrane filters. The Hly fusion protein in the supernatant was enriched by incubation with 100 μl butyl-Sepharose (Pharmacia) for 30 min at room temperature in a rotating device (Schoel et al., 1994). After centrifugation (3000 rpm) the pellet was dissolved in Laemmli buffer (Laemmli, 1970). Subsequently proteins were separated by 10% SDS/polyacrylamide gel electrophoresis as described previously (Laemmli, 1970) and were transferred to Hybond-PVDF membranes (Amersham Life Science). Immunostainings were performed with the anti-Hly mAb H14-3 (Nato et al., 1991) and peroxidase-conjugated secondary antibodies (Boehringer Mannheim). The washing procedure and the chemiluminescent immunodetection were performed according to the manufacturer's description [BM Western Blotting Kit (Mouse/Rabbit) (Boehringer Mannheim)]. The signal development on X-ray film (Kodak, XOMAT-AR) was performed for 1 min.

[0063] The hemolytic activity of supernatants and whole-bacterial suspensions of BCG pAT261:Hly, BCG pMV306Hly, BCG and L. monocytogenes were determined by serially diluting the samples in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin. Diluted samples (100 μl) were subsequently activated by adding cysteine (20 mM final concentration) and were incubated at 37° C. for 45 min with 50 μl of sheep erythrocytes (6×10⁸ cells/ml in PBS, pH 6.0) in 96-well plates, Hemolytic activities are complete CHU, which are defined as the reciprocal value of the highest dilution at which complete hemolysis was detectable (Gentschev et al., 1995).

[0064] 1.5 In Vitro Analysis of Mycobacterial Growth

[0065] Human and murine macrophage-like cells THP-1 (ATCC TIB-202) and J774A.1 (ATCC TIB-67), respectively, were allowed to adhere to 24-well plates (10⁶ per well). In case of THP-1, adherence was achieved by stimulation with 10 nM PMA (Sigma) 48 h prior to infection. Cells were infected at multiplicity of infection (moi) of 10 mycobacteria (BCG, BCG pAT261:Hly or BCG pMV306:Hly) per cell for 3 h. Immediately after infection, CFU were determined by plating serial dilutions of supernatants and cell lysates on 7H10 agar enriched with Bacto Middlebrook OADC (Difco) and appropriate 15 μg/ml kanamycin. The degree of mycobacterial uptake by macrophages were comparable. The remaining samples of infected macrophages were washed with PBS and further incubated for 14 days in the presence of 200 μg/ml gentamicin. Intracellular growth of recombinant BCG strains was determined by CFU analysis after 1, 8 or 15 days post infection (p.i.).

[0066] 1.6 LDH Release

[0067] The cytotoxicity of recombinant BCG strains and of L. monocytogenes EGD as positive control was determined by measuring the LDH release by infected J774A.1 macrophages. The culture supernatants and cell lysates of BCG, BCG pAT261.:Hly, BCG pMV306:Hly or L. monocytogenes EGD-infected J774A.1 macrophages were assayed for LDH activity using the quantitation kit obtained from Promega. J774A.1 cells (10⁴ per well) were seeded into 96-well plates and infected at moi of 10. One hour after infection, gentamicin (final concentration 200 μg/ml) was added to the samples, The LDH activity was quantitatively analysed at 3, 4, 5 or 24 h p.i. according to the manufacturer's instructions. The percentage of cytotoxicity was calculated as follows: % Cytotoxicity=(J774A.1 Infected-J774A.1 Spontaneous)/(J774A.1 Maximum-J774A.1 Spontaneous×100).

[0068] 2. Results

[0069] 2.1 Construction of the Mycobacteria—Escherichia coli Shuttle Expression Vectors pAT261:Hly and pMV306:Hly

[0070] In order to transfer the phagolysosomal escape function (mediated by Hly of L. monocytogenes EGD Sv 1/2a (Domann and Chakraborty, 1989)] to BCG Chicago two different E. coli-mycobacteria shuttle vectors pAT261 and pMV306 were used. The second-generation vector pAT261, a pMV261 derivative (Stover et al., 1991), directs extrachromosomal Hly expression with about five plasmid copies per BCG genome and the integrative plasmid pMV306, a derivation of pMV361, allows stable chromosomal expression of Hly (FIG. 1) (Stover et al., 1991).

[0071] A pILH-1 -derived 1.7 kb Pst I-DNA fragment coding for an hly-hlyA (E. coli pHly152-specific hemolysin A) open reading frame (ORF) was inserted into Pst I-site of plasmid pAT261 (Gentschev et al., 1995; Stover et al., 1993). This resulting gene fusion codes for the expression of secreted proteins directed to the supernatant by the BCG-specific Ag85B signal peptide (Matsuo et al., 1990). The construct was termed pAT261:Hly and its Xba I-Sal I DNA expression cassette under transcriptional control of the hsp60 mycobacterial promoter was subsequently used for insertion into the parental pMV306 vector resulting in the construct, pMV306:Hly (FIG. 1). The DNA sequence of the hly-specific insertion sites in both mycobacterial expression plamids, including the coding sequence for the BCG-specific Ag85B-signal peptide (Matsuo et al, 1990) was analysed. The derived amino acid sequence of the complete Hly fusion protein is presented in FIG. 2. The mature Hly fusion protein consists of 30 amino acids (aa) at the N-terminus and 52 aa at the C-terminal part of the fusion which originally belong to HlyA of E. coli (Gentschev et al., 1995).

[0072] Subsequently, each plasmid construct pAT261 .Hly or pMV306:Hly was electroporated into BCG Chicago strain resulting in BCG pAT261:Hly or BCG pMV306;Hly with plasmid or chromosomal Hly expression, respectively.

[0073] 2.2 Analysis of Hly Expression in BCG pAT261:Hly and BCG pMV306:Hly

[0074] To characterize Hly secretion by the BCG pAT261:Hly or by BCG pMV306:Hly strain appropriate supernatants and mycobacterial lysates of mid-logarithmic grown cultures were prepared according to Stover et al. (1993). The Hly fusion was enriched via hydrophobic interaction chromatography to overcome the observed cross-reactivity of anti-Hly monclonal antibodies (mAb) available for immunostaining (Schoel et al., 1994; Nato et al., 1991). The Hly fusion protein is detectable in lysates and supernatants of both mycobacterial strains, BCG pAT261:Hly and BCG pMV306:Hly (FIG. 3). The predicted size, 62 kDa, of the Hly-derived polypeptide is slightly larger than that of the original 58 kDa Hly protein of L. monocytogenes.

[0075] In order to characterize the pore-forming capacity of the Hly fusion protein, secreted by BCG pAT261:Hly and BCG pMV306:Hly, the hemolytic activity of whole-bacteria suspensions and of supernatants were determined. The samples of BCG pAT261:Hly and BCG pMV306:Hly reveal hemolytic activity on sheep erythrocytes (Table 1) which formally proves successful transfer of cytolytic Hly function to mycobacterial species. TABLE 1 Hemolytic activities of supernatant and whole-bacteria suspensions of recombinant BCG strains and L.monocytogenes EGD Hemolytic activity (CHU)^(a) Whole-bacteria Strain Supernatant suspension^(b) L.monocytogenes EGD 8 16 BCG pAT261:Hly 2 4 BCG pMV306:Hly 2 4 BCG ND^(c) ND

[0076] 2.3 Growth of Recombinant BCG Strains in Macrophages

[0077] Survival of BCG pAT261:Hly or BCG pMV306:Hly microorganisms in host cells was monitored by mycobacterial CFU of infected macrophages at day 1, 8 or 15 post infection (p.i.), The human monocytic cell line THP-1 (ATCC TIB-202) and the murine macrophage-like cell line J774A.1 (ATCC TIB-67) were used as mycobacterial target cells, Phorbol myristate acetate (PMA) stimulated THP-1 cells resemble native human monocyte-derived macrophages (Tsuchiya et al., 1982). Three hours after infection of THP-1 or J774A.1 cells the efficacy of mycobacterial phagocytosis was determined. Subsequent long term culture was performed in the presence of 200 μg/ml gentamicin to kill released or non-phagocytosed mycobacteria in the supernatant. As depicted in FIG. 4, each BCG strain, BCG pAT261:Hly and BCG pMV306:Hly, failed to grow in either type of host cell. Moreover, BCG pMV306:Hly bacteria showed impaired intracellular persistence in THP-1 and J774A.1 host cells as compared to the parental BCG strain. Noteworthy, the intracellular survival rate of BCG pMV306:Hly bacteria in THP-1 macrophages was already reduced at day 1 p.i. in regard to values of BCG or BCG pAT261:Hly-infected samples.

[0078] In contrast, the intracellular persistence of BCG pMV306:Hly was comparable to BCG in THP-1 (FIG. 4). Interestingly, at day 15 p.i. viable BCG pAT261:Hly bacteria were not detectable in infected J774A.1 cells suggesting complete growth inhibition of these mycobacterial constructs at least in the presence of gentamicin.

[0079] In order to gain insights into the impaired intracellular persistence of BCG pAT261:Hly and BCG pMV306:Hly strains, the cytotoxicity for J774A.1 macrophages of these recombinant BCG strains was determined in short term cultures. Cytotoxicity was analyzed by measuring lactate dehydrogenase (LDH) activity in supernatants of host cells infected with BCG; BCG pAT261:Hly; BCG pMV306:Hly, or L. monocytogenes EGD at 3, 4, 5 and 24 h p.i. At 24 h p.i, the amount of released LDH into supernatants did not significantly differ between parental BCG, BCG pAT261:Hly or BCG pMV306:Hly-infected and non-infected host cells (FIG. 4). In contrast, the fast-growing and hemolytic L. monocytogenes EGD strain caused profound LDH release into the supernatant within 24 h p.i. These data suggest that secretion of hemolytic Hly by recombinant BCG strains did not alter the cytotoxicity of the parental BCG strain. Rather, both BCG pAT261:Hly and BCG pMV306.Hly strains showed impaired persistence in murine macrophages as compared to the non-recombinant BCG carrier.

[0080] 2.4 Induction of Immune Responses by the Hly Recombinant BCG Strains in Mice

[0081] In order to analyze the immune responses elicted by the Hly recombinant BCG strains, C57BL/6 mice were immunized with the parental BCG strain or the two Hly secreting BCG strains, BCG pAT261-Hly and BCG pMV306-Hly. The IFN-γ production in supernatants of splenocytes restimulated with 1 μg BCG lysate was measured by ELISA at various time points. IFN-γ levels were increased in mice immunized with the Hly expressing BCG strains—BCG pAT261-Hly and BCG pMV306-Hly—compared to the parental BCG strain, with this effect being most pronounced in response to the recombinant BCG strains harbouring Hly as a chromosomal integrate (FIG. 5).

[0082] In order to test whether this increase in IFN-γ production was attributable to improved MHC class I presentation, frequencies of IFN-γ producing cells were analysed in ELispot assays, in which P815 cells were used for the presentation of CD8 specific Ag85A and Hsp65 epitopes. BALB/c mice (n=3) were immunized with the parental BCG strain (BCG) or the two Hly secreting BCG strains (BCG pAT261-Hly or BCG pMV306-Hly). At defined time points post immunization animals were sacrificed and spleens removed. Cultured splenocytes were restimulated with appropriate peptides (Ag85A-P15:QQFVYAGAMSGLLDFSQAMG, A111: FVGGQSSF, A:YAGAMSGL) and frequencies of specific CD8 T cells were determined in an IFN-γ ELispot). CO8T cells specific for mycobacterial antigens were detectable as early as 15 days after vaccination with hemolysin expressing BCG strains (FIG. 6).

[0083] 2.5 The Effects of Hly Expression on the Virulence of the Recombinant BCG Strains

[0084] The safety of the Hly expressing BCG recombinants was evaluated by following the course of infection in immunocompetent and immunodeficient mouse strains. C57BL/6 mice and TAP1 -deficient mice were immunized with 10⁶ CFU BCG (parental BCG strain) or BCG pMV308-Hly (BCG strain recombinant for listeriolysin) i.v,. Immunization with either the parental BCG strain or the BCG strain recombinant for listeriolysin revealed that hemolysin expression does not lead to increased virulence of these BCG strains (FIG. 7). Bacterial load in infected organs (spleen, liver, lung) was determined at time points up to 150 days post vaccination. No differences in colony forming units were observed in mice immunized with BCG or the BCG strain expressing hemolysin, in both C57BL/6 and TAP1-deficient animals. Comparable results were also obtained in β2m−/− mice. If any differences should be noted at all, a slight reduction in persistence of the recombinant strains might exist (FIG. 7). In addition, the results show that the recombinant vaccine strains are cleared on day 150 post immunization (FIG. 7) which is an essential piece of information for all challenge experiments.

[0085] 2.6 The Effect of Hemolysin Expression on the Protective Capacity of the BCG Strains

[0086] The initial experiment to assess the protective capacity of The hemolysin secreting BCG strains was performed by Dr. Ian Orme (Colorado State University, Fort Collins, Colo., USA) as part of the NIH vaccine project. C57BL/6 mice were immunized i.v. with 10⁶ CFU BCG (parental BCG strain) or the two Hly recombinants (BCG pAT261-Hly and BCG pMV306-Hly) and 10 weeks later they were challenged with M. tuberculosis H37Rv aerosol. Four weeks post infection the bacterial load in spleen and lung was determined. As depicted in FIG. 8, where the log10 protection of the three different strains is plotted, protection is marginally increased in the recombinant strains.

[0087] In addition, long term aerogenic challenge infections have been initiated in mice to monitor protection of the BCG strains beyond week 4 BALB/c and C57BL/6 mice were immunized i.v. with 2×10⁵ CFU BCG parental strain and Hly expressing BCG strain BCG pMV306-Hly and these animals were challenged with M. tuberculosis H37Rv via aerosol on day 150 post vaccination (ca. 150 CFU per lung). The course of the challenge infection has currently been monitored up to 6-7 months in addition to survival. The bacterial load in infected organs (spleen, liver, lung) is assessed at indicated time points post challenge. A schematic presentation of the experimental protocol of this still ongoing experiment is given in FIG. 9 A. In addition, the most recent results of the infection status (day 30 post challenge) of one mouse strain (BALB/c) is presented in FIG. 9 B. Immunization with the rBCG-Hly stain and the parental BCG Danish strain lead to a reduced bacterial load in infected organs compared with native animals. However, the course of M. tuberculosis infection does not significantly differ between the two BCG strains used for vaccination. At best, a retarded seeding of tubercle bacilli to the spleen of BCG pMV306-Hly vaccinated mice can be detected.

[0088] In a separate long term experiment, we have compared protection in immunocompetent and TAP-1 -deficient mice. The animals were immunized with hemolysin recombinant BCG (rBCG-Hly) or the parental strain (i.v. with 10⁶ CFU). Residual bacteria were cleared by rifampicin/isoniazid treatment 70 days after vaccination, before challenge with M. tuberculosis H37Rv by aerosol (ca, 150 CFU per lung). The bacterial load in infected organs (spleen, liver, lung) was assessed at 4 time points post challenge (day 30, 60, 98, 150), A schematic presentation of the experimental protocol of this still ongoing experiment is given in FIG. 10A.

[0089] If the protective capacity of the recombinant BCG strain was increased due to better stimulation of a CD8 T cell response, we would expect to see a difference in the course of the challenge infection in the wild-type mice but not the TAP-1-deficient mice, or alternatively, the difference in CFU between infected immunocompetent and TAP1-deficient mice should be greater in animals vaccinated with rBCG-Hly compared with the parental BCG strain, Although not blatantly obvious at first glance, a careful analysis of the obtained results reveals a trend that confirms our expectations. In the lungs, the Δlog of TAP1−/−0 versus C567BL/6 mice is greater in rBCG-Hly immunized animals than in mice vaccinated with the parental BCG strain (FIG. 10 B). In addition, particularly at a later time point post infection (day 98) the CFU in BCG pMV306-Hly immunized TAP1−/− mice as well as BCG Danish vaccinated C57BL/6 and TAP1-deficient animals are elevated in comparison with BCG pMV306-Hly immunized immunocompetent animals in systemic organs (liver, spleen). This suggests that in BCG pMV306-Hly vaccinated immunocompetent mice the additional stimulation of CD8 T cells contributes to protection.

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1 2 1 1881 DNA Artificial Sequence Description of Artificial Sequence recombinant nucleic acid molecule comprising a domain of Mycobacterium and a phagolysomal escape domain 1 atg aca gac gtg agc cga aag att cga gct tgg gga cgc cga ttg atg 48 Met Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu Met 1 5 10 15 atc ggc acg gca gcg gct gta gtc ctt ccg ggc ctg gtg ggg ctt gcc 96 Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu Ala 20 25 30 ggc gga gcg gca acc gcg ggc gcg ttc tcc cgg ccg ggg ctg ccg gtc 144 Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Arg Pro Gly Leu Pro Val 35 40 45 gag tac ctg cag tct gca aag caa tcc gct gca aat aaa ttg cac tca 192 Glu Tyr Leu Gln Ser Ala Lys Gln Ser Ala Ala Asn Lys Leu His Ser 50 55 60 gca gga caa agc acg aaa gat gca tct gca ttc aat aaa gaa aat tca 240 Ala Gly Gln Ser Thr Lys Asp Ala Ser Ala Phe Asn Lys Glu Asn Ser 65 70 75 80 att tca tcc atg gca cca cca gca tct ccg cct gca agt cct aag acg 288 Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser Pro Lys Thr 85 90 95 cca atc gaa aag aaa cac gcg gat gaa atc gat aag tat ata caa gga 336 Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr Ile Gln Gly 100 105 110 ttg gat tac aat aaa aac aat gta tta gta tac cac gga gat gca gtg 384 Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly Asp Ala Val 115 120 125 aca aat gtg ccg cca aga aaa ggt tac aaa gat gga aat gaa tat att 432 Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn Glu Tyr Ile 130 135 140 gtt gtg gag aaa aag aag aaa tcc atc aat caa aat aat gca gac att 480 Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn Ala Asp Ile 145 150 155 160 caa gtt gtg aat gca att tcg agc cta acc tat cca ggt gct ctc gta 528 Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly Ala Leu Val 165 170 175 aaa gcg aat tcg gaa tta gta gaa aat caa cca gat gtt ctc cct gta 576 Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val Leu Pro Val 180 185 190 aaa cgt gat tca tta aca ctc agc att gat ttg cca ggt atg act aat 624 Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly Met Thr Asn 195 200 205 caa gac aat aaa atc gtt gta aaa aat gcc act aaa tca aac gtt aac 672 Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser Asn Val Asn 210 215 220 aac gca gta aat aca tta gtg gaa aga tgg aat gaa aaa tat gct caa 720 Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys Tyr Ala Gln 225 230 235 240 gct tat cca aat gta agt gca aaa att gat tat gat gac gaa atg gct 768 Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp Glu Met Ala 245 250 255 tac agt gaa tca caa tta att gcg aaa ttt ggt aca gca ttt aaa gct 816 Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala Phe Lys Ala 260 265 270 gta aat aat agc ttg aat gta aac ttc ggc gca atc agt gaa ggg aaa 864 Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser Glu Gly Lys 275 280 285 atg caa gaa gaa gtc att agt ttt aaa caa att tac tat aac gtg aat 912 Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr Asn Val Asn 290 295 300 gtt aat gaa cct aca aga cct tcc aga ttt ttc ggc aaa gct gtt act 960 Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys Ala Val Thr 305 310 315 320 aaa gag cag ttg caa gcg ctt gga gtg aat gca gaa aat cct cct gca 1008 Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn Pro Pro Ala 325 330 335 tat atc tca agt gtg gcg tat ggc cgt caa gtt tat ttg aaa tta tca 1056 Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu Lys Leu Ser 340 345 350 act aat tcc cat agt act aaa gta aaa gct gct ttt gat gct gcc gta 1104 Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp Ala Ala Val 355 360 365 agc gga aaa tct gtc tca ggt gat gta gaa cta aca aat atc atc aaa 1152 Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn Ile Ile Lys 370 375 380 aat tct tcc ttc aaa gcc gta att tac gga ggt tcc gca aaa gat gaa 1200 Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala Lys Asp Glu 385 390 395 400 gtt caa atc atc gac ggc aac ctc gga gac tta cgc gat att ttg aaa 1248 Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp Ile Leu Lys 405 410 415 aaa ggc gct act ttt aat cga gaa aca cca gga gtt ccc att gct tat 1296 Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro Ile Ala Tyr 420 425 430 aca aca aac ttc cta aaa gac aat gaa tta gct gtt att aaa aac aac 1344 Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile Lys Asn Asn 435 440 445 tca gaa tat att gaa aca act tca aaa gct tat aca gat gga aaa att 1392 Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp Gly Lys Ile 450 455 460 aac atc gat cac tct gga gga tac gtt gct caa ttc aac att tct tgg 1440 Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn Ile Ser Trp 465 470 475 480 gat gaa gta aat tat gat cct gaa ggt aac gaa att gtt caa cat aaa 1488 Asp Glu Val Asn Tyr Asp Pro Glu Gly Asn Glu Ile Val Gln His Lys 485 490 495 aac tgg agc gaa aac aat aaa agc aag cta gct cat ttc aca tcg tcc 1536 Asn Trp Ser Glu Asn Asn Lys Ser Lys Leu Ala His Phe Thr Ser Ser 500 505 510 atc tat ttg cca ggt aac gcg aga aat att aat gtt tac gct aaa gaa 1584 Ile Tyr Leu Pro Gly Asn Ala Arg Asn Ile Asn Val Tyr Ala Lys Glu 515 520 525 tgc act ggt tta gct tgg gaa tgg tgg aga acg gta att gat gac cgg 1632 Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Thr Val Ile Asp Asp Arg 530 535 540 aac tta cca ctt gtg aaa aat aga aat atc tcc atc tgg ggc acc acg 1680 Asn Leu Pro Leu Val Lys Asn Arg Asn Ile Ser Ile Trp Gly Thr Thr 545 550 555 560 ctt tat ccg aaa tat agt aat aaa gta gat aat cca atc gaa tat gca 1728 Leu Tyr Pro Lys Tyr Ser Asn Lys Val Asp Asn Pro Ile Glu Tyr Ala 565 570 575 tta gcc tat gga agt cag ggt gat ctt aat cca tta att aat gaa atc 1776 Leu Ala Tyr Gly Ser Gln Gly Asp Leu Asn Pro Leu Ile Asn Glu Ile 580 585 590 agc aaa atc att tca gct gca gtt ctt tcc tct tta aca tcg aag cta 1824 Ser Lys Ile Ile Ser Ala Ala Val Leu Ser Ser Leu Thr Ser Lys Leu 595 600 605 cct gca gag ttc gtt agg cgc gga tcc gga att cga agc tta tcg atg 1872 Pro Ala Glu Phe Val Arg Arg Gly Ser Gly Ile Arg Ser Leu Ser Met 610 615 620 tcg acg tag 1881 Ser Thr 625 2 626 PRT Artificial Sequence Description of Artificial Sequence recombinant nucleic acid molecule comprising a domain of Mycobacterium and a phagolysomal escape domain 2 Met Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu Met 1 5 10 15 Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu Ala 20 25 30 Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Arg Pro Gly Leu Pro Val 35 40 45 Glu Tyr Leu Gln Ser Ala Lys Gln Ser Ala Ala Asn Lys Leu His Ser 50 55 60 Ala Gly Gln Ser Thr Lys Asp Ala Ser Ala Phe Asn Lys Glu Asn Ser 65 70 75 80 Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser Pro Lys Thr 85 90 95 Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr Ile Gln Gly 100 105 110 Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly Asp Ala Val 115 120 125 Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn Glu Tyr Ile 130 135 140 Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn Ala Asp Ile 145 150 155 160 Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly Ala Leu Val 165 170 175 Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val Leu Pro Val 180 185 190 Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly Met Thr Asn 195 200 205 Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser Asn Val Asn 210 215 220 Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys Tyr Ala Gln 225 230 235 240 Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp Glu Met Ala 245 250 255 Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala Phe Lys Ala 260 265 270 Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser Glu Gly Lys 275 280 285 Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr Asn Val Asn 290 295 300 Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys Ala Val Thr 305 310 315 320 Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn Pro Pro Ala 325 330 335 Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu Lys Leu Ser 340 345 350 Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp Ala Ala Val 355 360 365 Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn Ile Ile Lys 370 375 380 Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala Lys Asp Glu 385 390 395 400 Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp Ile Leu Lys 405 410 415 Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro Ile Ala Tyr 420 425 430 Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile Lys Asn Asn 435 440 445 Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp Gly Lys Ile 450 455 460 Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn Ile Ser Trp 465 470 475 480 Asp Glu Val Asn Tyr Asp Pro Glu Gly Asn Glu Ile Val Gln His Lys 485 490 495 Asn Trp Ser Glu Asn Asn Lys Ser Lys Leu Ala His Phe Thr Ser Ser 500 505 510 Ile Tyr Leu Pro Gly Asn Ala Arg Asn Ile Asn Val Tyr Ala Lys Glu 515 520 525 Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Thr Val Ile Asp Asp Arg 530 535 540 Asn Leu Pro Leu Val Lys Asn Arg Asn Ile Ser Ile Trp Gly Thr Thr 545 550 555 560 Leu Tyr Pro Lys Tyr Ser Asn Lys Val Asp Asn Pro Ile Glu Tyr Ala 565 570 575 Leu Ala Tyr Gly Ser Gln Gly Asp Leu Asn Pro Leu Ile Asn Glu Ile 580 585 590 Ser Lys Ile Ile Ser Ala Ala Val Leu Ser Ser Leu Thr Ser Lys Leu 595 600 605 Pro Ala Glu Phe Val Arg Arg Gly Ser Gly Ile Arg Ser Leu Ser Met 610 615 620 Ser Thr 625 

1. A recombinant nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is capable of eliciting an immune response in a mammal, and (b) a phagolysosomal escape domain.
 2. The nucleic acid according to claim 1, wherein said phagolysosomal escape domain is derived from an organism of the genus Listeria.
 3. The nucleic acid according to claim 1 or 2, wherein said phagolysosomal domain is encoded by a nucleic acid molecule selected from: (a) the nucleotide sequence from nucleotide 211-1722 as shown in SEQ ID No.1, (b) a nucleotide sequence which encodes for the same amino acid sequence as the sequence from (a), and (c) a nucleotide sequence hybridizing under stringent conditions with the sequence from (a) or (b).
 4. The nucleic acid according to any one of claims 1 to 3, wherein the domain capable of eliciting an immune response is a peptide or polypeptide capable of eliciting MHC class I-restricted CD8 T cell responses.
 5. The nucleic acid according to any one of claims 1 to 4, wherein the domain capable of eliciting an immune response is selected from the Mycobacterium antigens Ag85B (M. tuberculosis), Ag86B (M. bovis), so Ag85A (M. tuberculosis) and ESAT-6 (M. tuberculosis) or an immunogenic fragment thereof.
 6. The nucleic acid according to claim 5, wherein the domain capable of eliciting an immune response is the antigen Ag85 B or an immunogenic fragment thereof.
 7. The nucleic acid according to any one of claims 1 to 6, wherein the fusion polypeptide is preceded by a signal peptide sequence.
 8. The nucleic acid according to any one of claims 1 to 7, wherein a peptide linker is located between the immune response eliciting domain and the phagolysosomal domain.
 9. A recombinant vector comprising at least one copy of a nucleic acid molecule according to any one of claims 1 to
 8. 10. The vector according to claim 9, wherein said nucleic acid molecule is operatively linked with an expression control sequence.
 11. The vector according to claim 10, wherein said expression control sequence is active in Mycobacteria.
 12. The vector according to claim 9, 10 or 11, which is an extrachromosomal vector.
 13. The vector according to claim 9, 10 or 11, which is a chromosomal vector.
 14. A cell which comprises a recombinant nucleic acid molecule according to any one of claims 1 to 8 or a vector according to any one of claims 9 to
 13. 15. A recombinant Mycobacterium bovis cell, which comprises at least one recombinant nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain capable of eliciting an immune response in a mammal and (b) a phagolysosomal escape domain.
 16. A recombinant Mycobacterium bovis cell which comprises at least one recombinant nucleic acid molecule encoding a phagolysosomal escape peptide or polypeptide.
 17. The cell according to claim 16, which comprises at least one further recombinant nucleic acid molecule encoding a peptide or polypeptide capable of eliciting an immune response in a mammal.
 18. The cell according to claim 15 or 17, wherein the domain or peptide or polypeptide capable of eliciting an immune response is selected from autoantigens, tumor antigens, virus antigens, parasite antigens, bacterial antigens and immunogenic fragments thereof.
 19. The cell according to any one of claims 14 to 18, which is capable of expressing said at least one recombinant nucleic acid molecule.
 20. The cell according to any one of claims 14 or 19, which is capable of secreting a polypeptide encoded by said at least one nucleic acid molecule.
 21. The cell according to any one of claims 14 to 20, which has an intracellular persistence in infected macrophages which is equal or less than the intracellular persistence of a native Mycobacterium cell.
 22. Recombinant fusion polypeptide encoded by a nucleic acid molecule according to any one of claims 1 to
 8. 23. A pharmaceutical composition comprising as an active agent a cell according to any one of claims 14 to 21 or a polypeptide according to claim 22, optionally together with pharmaceutically acceptable diluents, carriers and adjuvants.
 24. A composition according to claim 23, which is a living vaccine suitable for administration to a mucosal surface or via the parenteral route.
 25. A method for the preparation of a living vaccine comprising formulating a cell according to any one of claims 14 to 21 in a pharmaceutically effective amount with pharmaceutically acceptable diluents, carriers and adjuvants.
 26. A method for preparing a recombinant bacterial cell according to claim 14 comprising the steps: (i) inserting a recombinant nucleic acid molecule into a bacterial cell, said nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is capable of eliciting an immune response in a mammal, and (b) a phagolysosomal escape domain, and (ii) cultivating the cell obtained according to (i) under suitable conditions.
 27. The method according to claim 25 or 26, wherein said cell is a M. bovis cell.
 28. A method for preparing a recombinant bacterial cell according to claim 15 comprising the steps: (i) inserting a recombinant nucleic acid molecule into a Mycobacterium bovis cells said nucleic acid molecule encoding a fusion polypeptide comprising (a) at least one domain from a polypeptide, wherein said domain is capable of eliciting an immune response in a mammal, and (b) a phagolysosomal escape domain, and (ii) cultivating the cell obtained according to (i) under suitable conditions.
 29. A method for preparing a recombinant bacterial cell according to claim 16 comprising the steps: (i) inserting a recombinant nucleic acid molecule into a Mycobacterium bovis cell, said nucleic acid molecule encoding a phagolysosomal escape peptide or polypeptide and (ii) cultivating the cell obtained according to (i) under suitable conditions.
 30. The method of claim 28 or 29 comprising inserting at least one further recombinant nucleic acid molecule into the Mycobacterium bovis cell, said further recombinant nucleic acid molecule encoding a peptide or polypeptide capable of eliciting an immune response in a mammal.
 31. The method according to claim 28 or 30, wherein the domain or peptide or polypeptide capable of eliciting an immune response is selected from autoantigens, tumor antigens, virus antigens, parasite antigens, bacterial antigens and immunogenic fragments thereof. 